Composite

Part:BBa_K2151202

Designed by: Pinja Hartikainen, Liam Pollock, James Provan   Group: iGEM16_Glasgow   (2016-08-30)


p25-crtEBIY

This biobrick was created through standard biobrick assembly of K2151001(p25; a moderate constitutive S. thermophilus promoter) K118014(RBS+crtE), K118006(RBS+crtB), K118005(RBS+crtI) and K118013(crtY). These genes are a part of the carotenoid biosynthesis pathway and together, this biobrick converts converts colourless farnesyl pyrophosphate to orange beta-carotene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2019
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1555
    Illegal NgoMIV site found at 1685
    Illegal AgeI site found at 770
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None